
蛋白质研究和抗体纯化用磁珠
为了能够快速有效地纯化带有标签的蛋白质和抗体,GenScript提供了超顺磁性的MagBeads系列磁珠。GenScript MagBeads偶联有重组蛋白配基,可与抗体和带标签的蛋白特异性地结合。磁株分离纯化方法可直接从细胞裂解物中纯化蛋白质,减少了离心步骤,提高了纯化效率。高结合能力,高结合特异性和超快的磁响应特性,使得GenScript MagBeads非常适合缩短纯化时间,及纯度大体积样品。
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应用领域
- 抗体纯化
- 标签蛋白纯化,如:His,GST,Flag和生物素等
- 高通量抗体和标签蛋白的纯化
Protein A、Protein G和Protein A/G 磁珠
标签蛋白纯化磁珠
应用 | 产品名称 | 原理 | 结合能力 | 规格 | 产品编号 |
---|---|---|---|---|---|
抗体纯化 | Protein A 磁珠 | 通过重组蛋白A或G,与不同物种IgG及其亚基的结合特性 | 10mg兔 IgG/ml磁珠 | 4ml | L00273 |
MagBeads MX Protein A 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00672 | ||
AmMag™ 耐碱Protein A 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00695 | ||
Protein G 磁珠 | 10mg山羊 IgG/ml磁珠 | 2ml | L00274 | ||
MagBeads MX Protein G 磁珠 | 25mg人 IgG/ml磁珠 | 4ml | L00673 | ||
Protein A/G 磁珠 | 10mg山羊或兔 IgG/ml磁珠 | 2ml | L00277 | ||
His标签蛋白纯化 | Ni-charged 磁珠 | 通过Ni2+捕获His标签蛋白 | 40mg 6×His标签蛋白 (27kD)/ml磁珠 | 8ml | L00295 |
AmMag™ Ni Magnetic beads | 通过带镍电荷的TED配体捕获His标签蛋白,耐EDTA和DTT | 10mg 6×His标签蛋白 (27kD)/ml磁珠 | 4/40/400ml | L00776 | |
Flag标签蛋白纯化 | MonoRab™ Anti-DYKDDDDK Magnetic Beads | 通过DYKDDDDK兔单抗捕获Flag标签蛋白 | 1.5mg DYKDDDDK标签蛋白(50KDa)/ml磁珠 | 1/5/25ml | L00835 |
GST融合蛋白纯化 | GST磁珠 | 通过还原型谷胱甘肽捕获GST融合蛋白 | 20 - 30mg GST/ml磁珠 | 8ml | L00327 |
生物素化样品分离纯化 | Streptavidin 磁珠 | 通过Streptavidin捕获生物素化的样品 | > 60nmol游离生物素/ml磁珠 | 2ml | L00424 |
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