365b体育下载质研究和抗体纯化用磁珠
为了能够快速有效地纯化带有标签的365b体育下载质和抗体，GenScript提供了超顺磁性的MagBeads系列磁珠。GenScript MagBeads偶联有重组365b体育下载配基，可与抗体和带标签的365b体育下载特异性地结合。磁株分离纯化方法可直接从细胞裂解物中纯化365b体育下载质，减少了离心步骤，提高了纯化效率。高结合能力，高结合特异性和超快的磁响应特性，使得GenScript MagBeads非常适合缩短纯化时间，及纯度大体积样品。
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应用领域
- 抗体纯化
- 标签365b体育下载纯化，如：His，GST，Flag和生物素等
- 高通量抗体和标签365b体育下载的纯化
Protein A、Protein G和Protein A/G 磁珠
标签365b体育下载纯化磁珠
| AmMag? Protein A Magnetic beads 动态载量 (L00695) | ||||
| Sample concentration (hIgG) | RT / 2 H | 37°C / Overnight | 4°C / 2 H | 4°C / Overnight |
|---|---|---|---|---|
| 0.01mg/ml | 2.5mg/ml | 5 | 2 | 2.5mg/ml |
| 0.1mg/ml | 20mg/ml | 30mg/ml | 15 | 20 |
| 0.5mg/ml | 35mg/ml | 60mg/ml | 30mg/ml | 50mg/ml |
| 1mg/ml | 45mg/ml | 70mg/ml | 40 | 60 |
| 3mg/ml | 70mg/ml | 100mg/ml | 60 | 85 |
| Ni charged Magbeads 动态载量 (L00295) | ||||
| Sample concentration (MBP) | RT / 2 H | 37C / Overnight | 4C / 2 H | 4C / Overnight |
|---|---|---|---|---|
| 0.01mg/ml | 2.5mg/ml | 2.5mg/ml | ||
| 0.1mg/ml | 30mg/ml | 20mg/ml | 10mg/ml | 20mg/ml |
| 0.5mg/ml | 50mg/ml | 40mg/ml | 30mg/ml | 40mg/ml |
| 2mg/ml | 60mg/ml | 50mg/ml | 40mg/ml | 50mg/ml |
| AmMag?Ni Magnetic Beads动态载量 (L00776) | |
| Sample concentration (MBP) | RT / 1 H |
|---|---|
| 0.01mg/ml | 1-2mg/ml |
| 0.1mg/ml | 8mg/ml |
| 0.5mg/ml | 9mg/ml |
| 1mg/ml | 9mg/ml |
| 应用 | 产品名称 | 原理 | 结合能力 | 规格 | 产品编号 |
|---|---|---|---|---|---|
| 抗体纯化 | Protein A 磁珠 | 通过重组365b体育下载A或G，与不同物种IgG及其亚基的结合特性 | 10mg兔 IgG/ml磁珠 | 4ml | L00273 |
| MagBeads MX Protein A 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00672 | ||
| AmMag™ 耐碱Protein A 磁珠 | 30mg人 IgG/ml磁珠 | 4ml | L00695 | ||
| Protein G 磁珠 | 10mg山羊 IgG/ml磁珠 | 2ml | L00274 | ||
| MagBeads MX Protein G 磁珠 | 25mg人 IgG/ml磁珠 | 4ml | L00673 | ||
| Protein A/G 磁珠 | 10mg山羊或兔 IgG/ml磁珠 | 2ml | L00277 | ||
| His标签365b体育下载纯化 | Ni-charged 磁珠 | 通过Ni2+捕获His标签365b体育下载 | 40mg 6×His标签365b体育下载 (27kD)/ml磁珠 | 8ml | L00295 |
| AmMag™ Ni Magnetic beads | 通过带镍电荷的TED配体捕获His标签365b体育下载，耐EDTA和DTT | 10mg 6×His标签365b体育下载 (27kD)/ml磁珠 | 4/40/400ml | L00776 | |
| Flag标签365b体育下载纯化 | MonoRab™ Anti-DYKDDDDK Magnetic Beads | 通过DYKDDDDK兔单抗捕获Flag标签365b体育下载 | 1.5mg DYKDDDDK标签365b体育下载（50KDa）/ml磁珠 | 1/5/25ml | L00835 |
| GST融合365b体育下载纯化 | GST磁珠 | 通过还原型谷胱甘肽捕获GST融合365b体育下载 | 20 - 30mg GST/ml磁珠 | 8ml | L00327 |
| 生物素化样品分离纯化 | Streptavidin 磁珠 | 通过Streptavidin捕获生物素化的样品 | > 60nmol游离生物素/ml磁珠 | 2ml | L00424 |
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